A method for detecting microfilaraemia, filarial specific antigens and antibodies and typing of parasites for drug resistance and genotypes using finger prick blood sample.
Identifieur interne : 006C41 ( Main/Exploration ); précédent : 006C40; suivant : 006C42A method for detecting microfilaraemia, filarial specific antigens and antibodies and typing of parasites for drug resistance and genotypes using finger prick blood sample.
Auteurs : S L Hoti [Inde] ; Rohit Sharma ; K Athisaya Mary ; R. Dhamodharan ; K. Krishnamoorthy ; P K DasSource :
- Acta tropica [ 1873-6254 ] ; 2008.
Descripteurs français
- KwdFr :
- Albendazole (pharmacologie), Animaux, Anticorps antihelminthe (sang), Antigènes d'helminthe (sang), Antihelminthiques (pharmacologie), Filariose lymphatique (diagnostic), Filariose lymphatique (parasitologie), Génotype, Humains, Profilage d'ADN, Réaction de polymérisation en chaîne (), Résistance aux substances, Sang (parasitologie), Technique RAPD, Test ELISA (), Wuchereria bancrofti (), Wuchereria bancrofti (génétique), Wuchereria bancrofti (immunologie), Wuchereria bancrofti (isolement et purification).
- MESH :
- diagnostic : Filariose lymphatique.
- génétique : Wuchereria bancrofti.
- immunologie : Wuchereria bancrofti.
- isolement et purification : Wuchereria bancrofti.
- parasitologie : Filariose lymphatique, Sang.
- pharmacologie : Albendazole, Antihelminthiques.
- sang : Anticorps antihelminthe, Antigènes d'helminthe.
- Animaux, Génotype, Humains, Profilage d'ADN, Réaction de polymérisation en chaîne, Résistance aux substances, Technique RAPD, Test ELISA, Wuchereria bancrofti.
English descriptors
- KwdEn :
- Albendazole (pharmacology), Animals, Anthelmintics (pharmacology), Antibodies, Helminth (blood), Antigens, Helminth (blood), Blood (parasitology), DNA Fingerprinting, Drug Resistance, Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (parasitology), Enzyme-Linked Immunosorbent Assay (methods), Genotype, Humans, Polymerase Chain Reaction (methods), Random Amplified Polymorphic DNA Technique, Wuchereria bancrofti (drug effects), Wuchereria bancrofti (genetics), Wuchereria bancrofti (immunology), Wuchereria bancrofti (isolation & purification).
- MESH :
- chemical , blood : Antibodies, Helminth, Antigens, Helminth.
- chemical , pharmacology : Albendazole, Anthelmintics.
- diagnosis : Elephantiasis, Filarial.
- drug effects : Wuchereria bancrofti.
- genetics : Wuchereria bancrofti.
- immunology : Wuchereria bancrofti.
- isolation & purification : Wuchereria bancrofti.
- methods : Enzyme-Linked Immunosorbent Assay, Polymerase Chain Reaction.
- parasitology : Blood, Elephantiasis, Filarial.
- Animals, DNA Fingerprinting, Drug Resistance, Genotype, Humans, Random Amplified Polymorphic DNA Technique.
Abstract
Monitoring and evaluation of programme to eliminate lymphatic filariasis (LF) depends on epidemiological assessment using appropriate indicators. Minimum efforts using reliable tests are necessary to guide the programme managers in decision-making. Impact of Mass Drug Administration (MDA) towards filariasis elimination can be assessed by the detection of microfilariae (mf) or parasite DNA (infective), filarial antigens (infected) and antibodies (exposure). It is also important to monitor drug resistance and variation in genetic structure of parasite populations using molecular markers. We developed a method to carry out parasitological, molecular, immunological and genetic analysis from a minimum volume of blood sample (about 150 microl) drawn from finger tip of an individual residing in LF endemic area. The method involves separation of sera for immunological assays and isolation of mf of Wuchereria bancrofti from the blood clots for counting, which were then used for W. bancrofti specific PCR, screening for albendazole sensitivity/resistance alleles by AS-PCR, RAPD profiling and ITS 2 PCR for genotyping. A protocol is also suggested for the separation of sera for assays to detect antigen and antibodies and isolation of mf from clots for genetic analysis. The protocol developed has shown potential application in monitoring several immunological, parasitological and molecular parameters from a limited amount of blood sample collected by finger prick, in large-scale operations.
DOI: 10.1016/j.actatropica.2008.07.011
PubMed: 18771650
Affiliations:
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Le document en format XML
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Dhamodharan</name></author><author><name sortKey="Krishnamoorthy, K" sort="Krishnamoorthy, K" uniqKey="Krishnamoorthy K" first="K" last="Krishnamoorthy">K. Krishnamoorthy</name></author><author><name sortKey="Das, P K" sort="Das, P K" uniqKey="Das P" first="P K" last="Das">P K Das</name></author></analytic><series><title level="j">Acta tropica</title><idno type="eISSN">1873-6254</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Albendazole (pharmacology)</term><term>Animals</term><term>Anthelmintics (pharmacology)</term><term>Antibodies, Helminth (blood)</term><term>Antigens, Helminth (blood)</term><term>Blood (parasitology)</term><term>DNA Fingerprinting</term><term>Drug Resistance</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (parasitology)</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Genotype</term><term>Humans</term><term>Polymerase Chain Reaction (methods)</term><term>Random Amplified Polymorphic DNA Technique</term><term>Wuchereria bancrofti (drug effects)</term><term>Wuchereria bancrofti (genetics)</term><term>Wuchereria bancrofti (immunology)</term><term>Wuchereria bancrofti (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Albendazole (pharmacologie)</term><term>Animaux</term><term>Anticorps antihelminthe (sang)</term><term>Antigènes d'helminthe (sang)</term><term>Antihelminthiques (pharmacologie)</term><term>Filariose lymphatique (diagnostic)</term><term>Filariose lymphatique (parasitologie)</term><term>Génotype</term><term>Humains</term><term>Profilage d'ADN</term><term>Réaction de polymérisation en chaîne ()</term><term>Résistance aux substances</term><term>Sang (parasitologie)</term><term>Technique RAPD</term><term>Test ELISA ()</term><term>Wuchereria bancrofti ()</term><term>Wuchereria bancrofti (génétique)</term><term>Wuchereria bancrofti (immunologie)</term><term>Wuchereria bancrofti (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Helminth</term><term>Antigens, Helminth</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Albendazole</term><term>Anthelmintics</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Filariose lymphatique</term><term>Sang</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Blood</term><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Albendazole</term><term>Antihelminthiques</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Antigènes d'helminthe</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>DNA Fingerprinting</term><term>Drug Resistance</term><term>Genotype</term><term>Humans</term><term>Random Amplified Polymorphic DNA Technique</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Génotype</term><term>Humains</term><term>Profilage d'ADN</term><term>Réaction de polymérisation en chaîne</term><term>Résistance aux substances</term><term>Technique RAPD</term><term>Test ELISA</term><term>Wuchereria bancrofti</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Monitoring and evaluation of programme to eliminate lymphatic filariasis (LF) depends on epidemiological assessment using appropriate indicators. Minimum efforts using reliable tests are necessary to guide the programme managers in decision-making. Impact of Mass Drug Administration (MDA) towards filariasis elimination can be assessed by the detection of microfilariae (mf) or parasite DNA (infective), filarial antigens (infected) and antibodies (exposure). It is also important to monitor drug resistance and variation in genetic structure of parasite populations using molecular markers. We developed a method to carry out parasitological, molecular, immunological and genetic analysis from a minimum volume of blood sample (about 150 microl) drawn from finger tip of an individual residing in LF endemic area. The method involves separation of sera for immunological assays and isolation of mf of Wuchereria bancrofti from the blood clots for counting, which were then used for W. bancrofti specific PCR, screening for albendazole sensitivity/resistance alleles by AS-PCR, RAPD profiling and ITS 2 PCR for genotyping. A protocol is also suggested for the separation of sera for assays to detect antigen and antibodies and isolation of mf from clots for genetic analysis. The protocol developed has shown potential application in monitoring several immunological, parasitological and molecular parameters from a limited amount of blood sample collected by finger prick, in large-scale operations.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Das, P K" sort="Das, P K" uniqKey="Das P" first="P K" last="Das">P K Das</name><name sortKey="Dhamodharan, R" sort="Dhamodharan, R" uniqKey="Dhamodharan R" first="R" last="Dhamodharan">R. Dhamodharan</name><name sortKey="Krishnamoorthy, K" sort="Krishnamoorthy, K" uniqKey="Krishnamoorthy K" first="K" last="Krishnamoorthy">K. Krishnamoorthy</name><name sortKey="Mary, K Athisaya" sort="Mary, K Athisaya" uniqKey="Mary K" first="K Athisaya" last="Mary">K Athisaya Mary</name><name sortKey="Sharma, Rohit" sort="Sharma, Rohit" uniqKey="Sharma R" first="Rohit" last="Sharma">Rohit Sharma</name></noCountry><country name="Inde"><noRegion><name sortKey="Hoti, S L" sort="Hoti, S L" uniqKey="Hoti S" first="S L" last="Hoti">S L Hoti</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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